RELION¶
RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class
averages in electron cryo-microscopy (cryo-EM).
Policy¶
RELION is freely available to users at HPC2N.
Citations
If you publish research that uses relion you have to cite it as follows:
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
or cite at least one of these papers:
- The underlying theory is given in Scheres (2012) JMB
- A more detailed description of its implementation is given in Scheres (2012) JSB
Overview¶
RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a software package that employs an empirical Bayesian approach for electron cryo-microscopy (cryo-EM) structure determination. It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology.
Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise.
The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) JSB.
A longer description of RELION can be found here on the RELION documentation page.
RELION at HPC2N¶
On HPC2N we have RELION available as a module on Kebnekaise. To see the available versions, login to Kebnekaise and do ml spider RELION.
Usage at HPC2N¶
To use, load the RELION module to add it to your environment. You give this command to see which versions are available of RELION:
and you then do
to see how to load a specific version and its prereqisites.
Example
RELION 4.0.0
and RELION 4.0.0 that is GPU enabled